The Apical Constriction Force of Madin-Darby Canine Kidney (MDCK) Cells
نویسندگان
چکیده
منابع مشابه
INTRODUCTION Endocytosis of ricin from the apical surface in polarized Madin-Darby canine kidney (MDCK) cells
Endocytosis of ricin from the apical surface in polarized Madin-Darby canine kidney (MDCK) cells seems to be under complex regulation (Fig. 1). Previous studies showed that apical clathrin-independent endocytosis is selectively increased by activation of protein kinase C (PKC) (Holm et al., 1995), protein kinase A (PKA) (Eker et al., 1994) and heterotrimeric G proteins (Eker et al., 1994). Unde...
متن کاملApical secretion of chondroitin sulphate in polarized Madin-Darby canine kidney (MDCK) cells.
Sugar moieties have been shown to contain sufficient and necessary information to target examples of secreted and transmembrane glycoproteins to the apical surface of epithelial MDCK cells. We have investigated if the sugar chains of proteoglycans, the glycosaminoglycans, also contain structural determinants for apical transport. Here we show that although 75% of the proteoglycan secretion from...
متن کاملFAPP2, cilium formation, and compartmentalization of the apical membrane in polarized Madin-Darby canine kidney (MDCK) cells.
We have analyzed the role of the phosphatidylinositol-4-phosphate adaptor protein-2 (FAPP2), a component of the apical transport machinery, in cilium formation in polarized Madin-Darby canine kidney (MDCK) cells. We show that ciliogenesis is defective in FAPP2 knockdown cells. Furthermore, by using fluorescence recovery after photobleaching studies of domain connectivity and the generalized pol...
متن کاملThe role of microtubules in apical and basolateral endocytosis in epithelial Madin-Darby canine kidney (MDCK) cells.
Introduction Studies on fluid and membrane endocytosis in simple epithelial cells have been performed on whole tissue [ 1-61 or with filter-grown epithelial cell lines [7-131. Both kinds of studies have demonstrated that endocytosis is initiated both at apical and basolateral surface domains of epithelia, and that the two endocytic pathways meet. However, the extent of meeting has been quantifi...
متن کاملRegulated Synthesis and Functions of Laminin 5 in Polarized Madin-Darby Canine Kidney (MDCK) Epithelial Cells
Renal tubular epithelial cells synthesize laminin 5 (LN5) during regeneration of the epithelium following ischemic injury. LN5 is a truncated laminin isoform of particular importance in the epidermis, but also constitutively expressed in a number of other epithelia. To investigate the role of LN5 in morphogenesis of a simple renal epithelium, we examined the synthesis and function of LN5 in the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2018
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.11.1814